Review




Structured Review

STEMCELL Technologies Inc easysep human cd14 positive selection kit ii
Easysep Human Cd14 Positive Selection Kit Ii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easysep+cd14+positive+selection+kit/easysep+human+t+cell+isolation+kit/pm40595560-402-12-8
Average 90 stars, based on 1 article reviews
easysep human cd14 positive selection kit ii - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

Suppression Assay:

Article Title: Recruitment of CCR2 + tumor associated macrophage to sites of liver metastasis confers a poor prognosis in human colorectal cancer
Article Snippet: Isolated cells were assessed for viability using 0.4% Trypan Blue Solution (Fisher Scientific) and manually counted. .. T-cell suppression assay Peripheral blood mononuclear cells (PBMC) and single cell suspensions of mCRC liver tumor from matched patients were used to extract CD14 + cells using the EasySep CD14 Positive Selection Kit per the manufacturer’s instructions (STEMCELL Technologies). .. CD3 + T-cells were isolated from the CD14 depleted PBMC using the EasySep CD3 Positive Selection Kit (STEMCELL Technologies) and labeled with carboxyfluorescein succinimidyl ester (CFSE; Life Technologies).

Single Cell:

Article Title: Recruitment of CCR2 + tumor associated macrophage to sites of liver metastasis confers a poor prognosis in human colorectal cancer
Article Snippet: Isolated cells were assessed for viability using 0.4% Trypan Blue Solution (Fisher Scientific) and manually counted. .. T-cell suppression assay Peripheral blood mononuclear cells (PBMC) and single cell suspensions of mCRC liver tumor from matched patients were used to extract CD14 + cells using the EasySep CD14 Positive Selection Kit per the manufacturer’s instructions (STEMCELL Technologies). .. CD3 + T-cells were isolated from the CD14 depleted PBMC using the EasySep CD3 Positive Selection Kit (STEMCELL Technologies) and labeled with carboxyfluorescein succinimidyl ester (CFSE; Life Technologies).

Selection:

Article Title: Recruitment of CCR2 + tumor associated macrophage to sites of liver metastasis confers a poor prognosis in human colorectal cancer
Article Snippet: Isolated cells were assessed for viability using 0.4% Trypan Blue Solution (Fisher Scientific) and manually counted. .. T-cell suppression assay Peripheral blood mononuclear cells (PBMC) and single cell suspensions of mCRC liver tumor from matched patients were used to extract CD14 + cells using the EasySep CD14 Positive Selection Kit per the manufacturer’s instructions (STEMCELL Technologies). .. CD3 + T-cells were isolated from the CD14 depleted PBMC using the EasySep CD3 Positive Selection Kit (STEMCELL Technologies) and labeled with carboxyfluorescein succinimidyl ester (CFSE; Life Technologies).

Article Title: Identification of cross-reactive CD8 + T cell receptors with high functional avidity to a SARS-CoV-2 immunodominant epitope and its natural mutant variants.
Article Snippet: .. Monocytes were enriched from the PBMCs of healthy individuals by using the EasySep CD14 positive selection kit according to the manufacturer’s instructions (STEMCELL Technologies, Canada). .. Monocytes were cultured in the DC medium for 6 days, which was made with RPMI-1640 medium supplemented with 10% FBS, 100 IU/ml penicillin, 100 mg/ml streptomycin, 2 mM L-glutamine (Gibco, USA), 800 IU/ml GM-CSF (PeproTech, USA) and 800 IU/ml IL-4 (PeproTech, USA).

Article Title: Leptin enhances the secretion of interleukin (IL)-18, but not IL-1β, from human monocytes via activation of caspase-1.
Article Snippet: Circulating levels of leptin are elevated in type-2 diabetes mellitus (T2DM) and leptin plays a role in immune responses.. Elevated circulating IL-18 levels are associated with clinical complications of T2DM.. IL-18 regulates cytokine secretion and the function of a number of immune cells including T-cells, neutrophils and macrophages and as such has a key role in immunity and inflammation.

Article Title: Identification of an HLA-A*11:01-restricted neoepitope of mutant PIK3CA and its specific T cell receptors for cancer immunotherapy targeting hotspot driver mutations
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated by density-gradient centrifugation using lymphocyte separation medium (Lymphoprep, STEMCELL) and cryopreserved until ready for use. .. To generate autologous monocyte-derived dendritic cells (moDCs), the monocytes were enriched from the PBMCs of healthy individuals by using the EasySep CD14 positive selection kit according to the manufacturer’s instructions (STEMCELL). ..

Article Title: Identification of cross-reactive CD8 + T cell receptors with high functional avidity to a SARS-CoV-2 immunodominant epitope and its natural mutant variants
Article Snippet: .. Monocytes were enriched from the PBMCs of healthy individuals by using the EasySep CD14 positive selection kit according to the manufacturer's instructions (STEMCELL Technologies, Canada). .. Monocytes were cultured in the DC medium for 6 days, which was made with RPMI-1640 medium supplemented with 10% FBS, 100 IU/ml penicillin, 100 μg/ml streptomycin, 2 mM l -glutamine (Gibco, USA), 800 IU/ml GM-CSF (PeproTech, USA) and 800 IU/ml IL-4 (PeproTech, USA).

Article Title: Expansion of the CD4 + effector T cell repertoire characterizes peanut-allergic patients with heightened clinical sensitivity
Article Snippet: .. Cell culture for secreted cytokine analysis Cryopreserved PBMC were thawed and monocytes and memory CD4 + T cells were isolated using the EasySep CD14 positive selection kit and memory CD4 + T cell enrichment kit, respectively (Stemcell Technologies). .. Autologous monocytes and T cells were cocultured in a 1: 1 ratio (6×10 5 monocytes and 6×10 5 memory CD4 + T cells) in 0.5 ml AIM V medium per well in a 48-well plate for 3 days, and were left unstimulated or cultured with 50 μg/ml peanut extract or T-Activator CD3/CD28 beads (Thermo Fisher) in a 1:10 ratio of beads to T cells.

Article Title: Major transcriptional changes observed in the Fulani, an ethnic group less susceptible to malaria
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were purified by Ficoll-Paque density centrifugation according to manufacturer’s instruction (GE Healthcare, Uppsala, Sweden). .. CD14+ monocytes were isolated with EasySep CD14 positive selection kit according to manufacturer’s instructions (Stem Cell Technologies, Grenoble, France). .. CD14+ monocytes and the remaining CD14- cells (lymphocytes) were stored in RNAprotect Cell Reagent (Qiagen, Hilden, Germany) at 80 ̊C (Materials and Methods - Supplement Figure 1).

Article Title: Expansion of the CD4 + effector T cell repertoire characterizes peanut-allergic patients with heightened clinical sensitivity
Article Snippet: .. Cryopreserved PBMC were thawed and monocytes and memory CD4 + T cells were isolated using the EasySep CD14 positive selection kit and memory CD4 + T cell enrichment kit, respectively (Stemcell Technologies). .. Autologous monocytes and T cells were cocultured in a 1: 1 ratio (6×10 5 monocytes and 6×10 5 memory CD4 + T cells) in 0.5 ml AIM V medium per well in a 48-well plate for 3 days, and were left unstimulated or cultured with 50 μg/ml peanut extract or T-Activator CD3/CD28 beads (Thermo Fisher) in a 1:10 ratio of beads to T cells.

Isolation:

Article Title: Leptin enhances the secretion of interleukin (IL)-18, but not IL-1β, from human monocytes via activation of caspase-1.
Article Snippet: Circulating levels of leptin are elevated in type-2 diabetes mellitus (T2DM) and leptin plays a role in immune responses.. Elevated circulating IL-18 levels are associated with clinical complications of T2DM.. IL-18 regulates cytokine secretion and the function of a number of immune cells including T-cells, neutrophils and macrophages and as such has a key role in immunity and inflammation.

Article Title: Expansion of the CD4 + effector T cell repertoire characterizes peanut-allergic patients with heightened clinical sensitivity
Article Snippet: .. Cell culture for secreted cytokine analysis Cryopreserved PBMC were thawed and monocytes and memory CD4 + T cells were isolated using the EasySep CD14 positive selection kit and memory CD4 + T cell enrichment kit, respectively (Stemcell Technologies). .. Autologous monocytes and T cells were cocultured in a 1: 1 ratio (6×10 5 monocytes and 6×10 5 memory CD4 + T cells) in 0.5 ml AIM V medium per well in a 48-well plate for 3 days, and were left unstimulated or cultured with 50 μg/ml peanut extract or T-Activator CD3/CD28 beads (Thermo Fisher) in a 1:10 ratio of beads to T cells.

Article Title: Major transcriptional changes observed in the Fulani, an ethnic group less susceptible to malaria
Article Snippet: Peripheral blood mononuclear cells (PBMCs) were purified by Ficoll-Paque density centrifugation according to manufacturer’s instruction (GE Healthcare, Uppsala, Sweden). .. CD14+ monocytes were isolated with EasySep CD14 positive selection kit according to manufacturer’s instructions (Stem Cell Technologies, Grenoble, France). .. CD14+ monocytes and the remaining CD14- cells (lymphocytes) were stored in RNAprotect Cell Reagent (Qiagen, Hilden, Germany) at 80 ̊C (Materials and Methods - Supplement Figure 1).

Article Title: Expansion of the CD4 + effector T cell repertoire characterizes peanut-allergic patients with heightened clinical sensitivity
Article Snippet: .. Cryopreserved PBMC were thawed and monocytes and memory CD4 + T cells were isolated using the EasySep CD14 positive selection kit and memory CD4 + T cell enrichment kit, respectively (Stemcell Technologies). .. Autologous monocytes and T cells were cocultured in a 1: 1 ratio (6×10 5 monocytes and 6×10 5 memory CD4 + T cells) in 0.5 ml AIM V medium per well in a 48-well plate for 3 days, and were left unstimulated or cultured with 50 μg/ml peanut extract or T-Activator CD3/CD28 beads (Thermo Fisher) in a 1:10 ratio of beads to T cells.

Centrifugation:

Article Title: Leptin enhances the secretion of interleukin (IL)-18, but not IL-1β, from human monocytes via activation of caspase-1.
Article Snippet: Circulating levels of leptin are elevated in type-2 diabetes mellitus (T2DM) and leptin plays a role in immune responses.. Elevated circulating IL-18 levels are associated with clinical complications of T2DM.. IL-18 regulates cytokine secretion and the function of a number of immune cells including T-cells, neutrophils and macrophages and as such has a key role in immunity and inflammation.

Cell Culture:

Article Title: Expansion of the CD4 + effector T cell repertoire characterizes peanut-allergic patients with heightened clinical sensitivity
Article Snippet: .. Cell culture for secreted cytokine analysis Cryopreserved PBMC were thawed and monocytes and memory CD4 + T cells were isolated using the EasySep CD14 positive selection kit and memory CD4 + T cell enrichment kit, respectively (Stemcell Technologies). .. Autologous monocytes and T cells were cocultured in a 1: 1 ratio (6×10 5 monocytes and 6×10 5 memory CD4 + T cells) in 0.5 ml AIM V medium per well in a 48-well plate for 3 days, and were left unstimulated or cultured with 50 μg/ml peanut extract or T-Activator CD3/CD28 beads (Thermo Fisher) in a 1:10 ratio of beads to T cells.



Similar Products

90
STEMCELL Technologies Inc easysep human cd14 positive selection kit ii
Easysep Human Cd14 Positive Selection Kit Ii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easysep+cd14+positive+selection+kit/easysep+human+t+cell+isolation+kit/pm40595560-402-12-8
Average 90 stars, based on 1 article reviews
easysep human cd14 positive selection kit ii - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
STEMCELL Technologies Inc easysep human cd14 positive selection kit
Easysep Human Cd14 Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easysep+cd14+positive+selection+kit/easysep+human+t+cell+isolation+kit/pm40505138-355-7-13
Average 90 stars, based on 1 article reviews
easysep human cd14 positive selection kit - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
STEMCELL Technologies Inc monocyte isolation easysep human cd14 positive selection kit ii
Monocyte Isolation Easysep Human Cd14 Positive Selection Kit Ii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easysep+cd14+positive+selection+kit/rosettesep+ab+mixture/pmc12176863-34-10-20
Average 90 stars, based on 1 article reviews
monocyte isolation easysep human cd14 positive selection kit ii - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
STEMCELL Technologies Inc easysep human cd14 positive select kit ii
Easysep Human Cd14 Positive Select Kit Ii, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easysep+cd14+positive+selection+kit/easysep+human+t+cell+isolation+kit/pm40216442-66-33-40
Average 90 stars, based on 1 article reviews
easysep human cd14 positive select kit ii - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results